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Avidity-based Extracellular Interaction Screening (AVEXIS) for the Scalable Detection of Low-affinity Extracellular Receptor-Ligand Interactions
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Avidity-based Extracellular Interaction Screening (AVEXIS) for the Scalable Detection of Low-affinity Extracellular Receptor-Ligand Interactions

Avidity-based Extracellular Interaction Screening (AVEXIS) for the Scalable Detection of Low-affinity Extracellular Receptor-Ligand Interactions

DOI:
10.3791/3881-v

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12:30 min

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March 05, 2012

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1Cell Surface Signalling Laboratory,Wellcome Trust Sanger Institute

Chapters

  • 00:05Title
  • 03:50Prey- and Bait-protein Expression
  • 05:19Normalization of the Bait Samples
  • 08:21Prey Normalization
  • 09:13AVEXIS Screening
  • 10:43Representative Results
  • 11:20Conclusion

Summary

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AVEXIS is a high throughput protein interaction assay developed to systematically screen for novel extracellular receptor-ligand pairs involved in cellular recognition processes. It is specifically designed to detect transient protein interactions that are difficult to identify using other high throughput approaches.

Tags

Avidity-based Extracellular Interaction ScreeningAVEXISLow-affinity InteractionsExtracellular Receptor-ligand InteractionsIntercellular CommunicationMulticellular OrganismsProtein-protein InteractionsBinding PartnerMembrane-embedded ProteinsPosttranslational ModificationsInteraction AffinitiesHigh Throughput MethodsAVEXIS AssayWeak Protein InteractionsFalse Positive RateHigh Throughput FormatRecombinant Protein Libraries

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