Electrostatic Force Microscopy

Electrostatic Force Microscopy (EFM) is a scanning probe technique that maps electrical forces, surface charge, and potential variations at the nanoscale, making it valuable for studying materials and devices in physics. A conductive atomic force microscope tip is biased relative to the sample, and electrostatic interactions alter the cantilever’s deflection, oscillation amplitude, or resonance behavior; these changes are recorded as the tip scans the surface, often at a controlled lift height. EFM helps characterize semiconductors, dielectric films, nanostructures, and charge-trapping behavior, supporting research in electronic materials, device failure analysis, and nanoscale energy and transport phenomena.

Electrostatic Force Microscopy - Related Videos

Research

JoVE Journal - Bioengineering

Atomic Force Microscopy Imaging and Force Spectroscopy of Supported Lipid Bilayers

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Cited by 34 •

2015

We describe a protocol for preparation of supported lipid bilayers and its characterization using atomic force microscopy and force spectroscopy.

Bacterial Immobilization for Imaging by Atomic Force Microscopy

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Cited by 36 •

2011

Live Gram-negative and Gram-positive bacteria can be immobilized on gelatin-coated mica and imaged in liquid using Atomic Force Microscopy (AFM).

Static Atomic Force Microscopy to Visualize and Characterize Assembled Nucleosomes

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2025

In this video, we demonstrate static atomic force microscopy to visualize assembled nucleosomes. This high-resolution imaging technique allows the study of the nucleosome structure.

Education

JoVE Core - Cell Biology

Atomic Force Microscopy

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2023

Atomic force microscopy (AFM) is a type of scanning probe microscopy that can analyze topographic details of various specimens like ceramics, glass, polymers, and biological samples. AFM offers over 1000 times more resolution than the optical imaging system. Images generated from AFM are three-dimensional surface profiles, offering an advantage over the flat, two-dimensional images from other imaging techniques. The AFM Probe The probe is regarded as the heart of any AFM setup and comprises the...

Intermolecular Forces

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2020

Atoms and molecules interact through bonds (or forces): intramolecular and intermolecular. The forces are electrostatic as they arise from interactions (attractive or repulsive) between charged species (permanent, partial, or temporary charges) and exist with varying strengths between ions, polar, nonpolar, and neutral molecules. The different types of intermolecular forces are ion–dipole, dipole–dipole, hydrogen bonds, and dispersion; among these, dipole–dipole, hydrogen bonds, and dispersion...

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