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Bacterial Immobilization for Imaging by Atomic Force Microscopy

JoVE Journal
Bioengineering

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JoVE Journal Bioengineering

Bacterial Immobilization for Imaging by Atomic Force Microscopy

Bacterial Immobilization for Imaging by Atomic Force Microscopy

DOI:

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10:03 min

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August 10, 2011

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David P. Allison², Claretta J. Sullivan, Ninell Pollas Mortensen², Scott T. Retterer⁴, Mitchel Doktycz⁴

¹Biological and Nanoscale Systems Group, Biosciences Division,Oak Ridge National Laboratory, ²Department of Biochemistry and Cellular and Molecular Biology,University of Tennessee , ³Department of Surgery,Eastern Virginia Medical School, ⁴Center for Nanophase Materials Sciences Division,Oak Ridge National Laboratory

Chapters

00:05Title
02:07Preparation of Gelatin Coated Mica
04:13Mounting Bacteria on Gelatin Coated Mica
06:48Atomic Force Microscopy
08:11Representative Results
08:55Conclusion

Summary

Automatic Translation

Live Gram-negative and Gram-positive bacteria can be immobilized on gelatin-coated mica and imaged in liquid using Atomic Force Microscopy (AFM).

Tags

Bacterial Immobilization Atomic Force Microscopy High-resolution Imaging Imaging Cells Biomolecules Liquid Environment Scanning AFM Cantilever Tip Sample Immobilization Minimally Invasive Immobilization Porcine Gelatin Coating Negative Bacteria Electrostatic Interaction Factors Affecting Immobilization Microbial Cell Imaging

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