Tuning Fork

A tuning fork is a U-shaped metal instrument designed to produce a stable sound at a characteristic natural frequency, making it a useful model for studying vibration and resonance in physics. When struck, its two prongs vibrate in opposite directions, while the stem and surrounding air transmit periodic pressure variations as sound waves; the fork’s geometry and material determine its frequency, and energy loss gradually damps the motion. Tuning forks provide reliable reference frequencies for calibrating instruments, demonstrating forced vibration and resonance, and investigating wave behavior. They also support measurements of frequency and speed of sound in laboratory experiments.

Tuning Fork - Related Videos

Education

JoVE Core - Molecular Biology
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Restarting Stalled Replication Forks

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2020

DNA replication is initiated at sites containing predefined DNA sequences known as origins of replication. DNA is unwound at these sites by the minichromosome maintenance (MCM) helicase and other factors such as Cdc45 and the associated GINS complex.The unwound single strands are protected by replication protein A (RPA) until DNA polymerase starts synthesizing DNA at the 5’ end of the strand in the same direction as the replication fork. To prevent the replication fork from falling apart, a...

Education

JoVE Core - Cell Biology
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Restarting Stalled Replication Forks

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2023

DNA replication is initiated at sites containing predefined DNA sequences known as origins of replication. DNA is unwound at these sites by the minichromosome maintenance (MCM) helicase and other factors such as Cdc45 and the associated GINS complex.The unwound single strands are protected by replication protein A (RPA) until DNA polymerase starts synthesizing DNA at the 5’ end of the strand in the same direction as the replication fork. To prevent the replication fork from falling apart, a...

Education

JoVE Core - Molecular Biology

The DNA Replication Fork

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2020

An organism’s genome needs to be duplicated in an efficient and error-free manner for its growth and survival. The replication fork is a Y-shaped active region where two strands of DNA are separated and replicated continuously. The coupling of DNA unzipping and complementary strand synthesis is a characteristic feature of a replication fork. Organisms with small circular DNA, such as E. coli, often have a single origin of replication; therefore, they have only two replication forks, one in...

Education

JoVE Core - Cell Biology
Free Sample

The DNA Replication Fork

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2023

An organism’s genome needs to be duplicated in an efficient and error-free manner for its growth and survival. The replication fork is a Y-shaped active region where two strands of DNA are separated and replicated continuously. The coupling of DNA unzipping and complementary strand synthesis is a characteristic feature of a replication fork. Organisms with small circular DNA, such as E. coli, often have a single origin of replication; therefore, they have only two replication forks, one in...

Research

JoVE Journal - Biology
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Direct Restart of a Replication Fork Stalled by a Head-On RNA Polymerase

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Cited by 11 •

2010

The fate of the replisome following a collision with a head-on RNA polymerase (RNAP) is unknown. We find that the replisome stalls upon collision with a head-on RNAP, but resumes elongation after displacing the RNAP from DNA. Mfd promotes replication restart by facilitating displacement of the RNAP after the collision.

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