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Differentiation of Embryonic Stem Cells into Oligodendrocyte Precursors

Article DOI: 10.3791/1960-v • 08:11 min • May 19th, 2010
May 19th, 2010 •
1, 1, 1
1Department of Cell Biology and Human Anatomy Institute for Pediatric Regenerative Medicine, School of Medicine, University of California, Davis

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We describe a small molecule-based protocol for differentiation of mouse embryonic stem cells into oligodendrocyte precursor cells (OPCs). This protocol generates Olig2+NG2+ OPCs with high efficiency by 30 days of differentiation. We also describe a method to generate "spiking" OPCs that can fire action potentials.

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Embryonic Stem Cells Oligodendrocyte Precursors Myelinating Cells Central Nervous System Regenerative Cell Therapy Demyelinating Diseases Transplantation Transcription Factor Olig2 Proteoglycan NG2 GFP-Olig2 Mouse Embryonic Stem Cell Reporter Line Differentiation Small Molecules Retinoic Acid Sonic Hedgehog Agonist Purmorphamine Electrophysiological Property NaV1.2 Subunit
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