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Generation of RNA/DNA Hybrids in Genomic DNA by Transformation using RNA-containing Oligonucleotides

Article DOI: 10.3791/2152-v • 16:42 min • November 24th, 2010
November 24th, 2010 •
1, 1
1School of Biology, Georgia Institute of Technology

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This work shows how to form an RNA/DNA hybrid at the chromosomal level and reveal transfer of genetic information from RNA to genomic DNA in yeast cells.

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RNA/DNA Hybrids Genomic DNA Transformation RNA-containing Oligonucleotides Synthetic Short Nucleic Acid Polymers Oligonucleotides Molecular Biology Tools Base And Sugar Modifications Nucleic Acid Alterations DNA Damage Mechanisms Of Repair Thermo Scientific Dharmacon RNA-containing Oligos In Vivo Study Chromosomal RNA/DNA Hybrids Ribonucleotides Embedded Into DNA Genetic Integrity Of Cells Ribonucleotide Incorporation Eukaryotic Model System Yeast/Saccharomyces Cerevisiae
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