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The Method of Rodent Whole Embryo Culture using the Rotator-type Bottle Culture System

Article DOI: 10.3791/2170-v • 10:05 min • August 28th, 2010
August 28th, 2010 •
1, 1,2
1Division of Developmental Neuroscience, United Centers for Advanced Research and Translational Medicine (ART), Tohoku University Graduate School of Medicine, 2The Core Research for Evolutional Science and Technology (CREST), Japan Science and Technology Corporation (JST)

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résumé
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Whole embryo culture technique allows us to culture mouse and rat embryos ex vivo condition during limited periods corresponding to midgestation stages. In this video protocol, we demonstrate our standard procedures of rat whole embryo culture after E12.5 using the rotator-type bottle culture system.

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Rodent Whole Embryo Culture WEC Technique Rotator-type Bottle Culture System Developmental Biology Mouse Embryos Rat Embryos Ex Vivo Condition Embryonic Day Placenta Function Manipulation Under Microscope Dynamic Developmental Processes Postimplanted Mammalian Embryos Long-term Culture Rat WEC After E12.5 Refined Model Applications In Mammalian Developmental Biology
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