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Immunology and Infection

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JoVE Journal Immunology and Infection
Quantitative Assessment of Immune Cells in the Injured Spinal Cord Tissue by Flow Cytometry
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Quantitative Assessment of Immune Cells in the Injured Spinal Cord Tissue by Flow Cytometry: a Novel Use for a Cell Purification Method

Article DOI: 10.3791/2698-v • 08:01 min • April 9th, 2011
April 9th, 2011 •
1,2,3,4, 5, 1,2,3,4,6
1Institute for Memory Impairments and Neurological Disorders, University of California, 2Physical Medicine & Rehabilitation, University of California, 3Anatomy & Neurobiology, University of California, 4Sue and Bill Gross Stem Cell Research Center, University of California, 5Section of Molecular Biology, University of California, 6Reeve-Irvine Research Center, University of California

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résumé
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Quantification of cellular inflammation in the injured/pathological CNS by flow cytometry is complicated by lipid/myelin debris that can have similar size and granulation to cells, decreasing sensitivity/accuracy. We have advanced a cell preparation method to remove myelin debris and improve cell detection by flow cytometry in the injured spinal cord.

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Quantitative Assessment Immune Cells Injured Spinal Cord Tissue Flow Cytometry Cell Purification Method Central Nervous System (CNS) Cellular Inflammation Morphological Techniques Histological Techniques Alternative Method Brain Tissue Dissociated Cord Tissue Myelin Debris OptiPrep Gradient System Sensitive Quantifications Reliable Quantifications Injury Severity Characterization
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