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Isolation and Expansion of Human Glioblastoma Multiforme Tumor Cells Using the Neurosphere Assay
Journal JoVE
Neurosciences
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Journal JoVE Neurosciences
Isolation and Expansion of Human Glioblastoma Multiforme Tumor Cells Using the Neurosphere Assay

Isolation and Expansion of Human Glioblastoma Multiforme Tumor Cells Using the Neurosphere Assay

DOI:
10.3791/3633-v

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09:24 min

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October 30, 2011

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, , , , ,
1Department of Neurosurgery,University of Florida , 2Department of Anatomical Sciences,Shiraz University of Medical Sciences

Chapitres

  • 00:05Titre
  • 00:26Introduction
  • 00:49Primary GBM Neurosphere Culture
  • 05:06Passaging GBM-Derived Neurospheres
  • 07:43Representative Results
  • 08:41Conclusion

Summary

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This video protocol demonstrates the isolation and expansion of stem like cells from surgically resected human glioblastoma mutliforme (GBM) tumor tissue using the neurosphere assay culture method.

Tags

IsolationExpansionHuman Glioblastoma MultiformeTumor CellsNeurosphere AssayStem-like CellsBreastLungColonProstateBrainTumor Initiating Cell Population(s)Tumor FormationProgressionRecurrenceTherapeutic ApproachesNeurosphere Culture MethodChemical DigestionMechanical DissociationSingle Cell SuspensionNSA CulturePrimary Neurospheres

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