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Monitoring Plasmid Replication in Live Mammalian Cells over Multiple Generations by Fluorescence Microscopy

Article DOI: 10.3791/4305-v • 12:34 min • December 13th, 2012
December 13th, 2012 •
1, 1, 1
1Department of Oncology, University of Wisconsin - Madison

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A method of observing individual DNA molecules in live cells is described. The technique is based on the binding of a fluorescently tagged lac repressor protein to binding sites engineered into the DNA of interest. This method can be adapted to follow many recombinant DNAs in live cells over time.

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Plasmid Replication Live Mammalian Cells Fluorescence Microscopy Gamma-herpesviruses Epstein-Barr Virus Kaposi's Sarcoma-associated Herpesvirus Licensed Replication Viral Protein Cellular Replication Machinery Centromeres Southern Blotting Fluorescence In Situ Hybridization (FISH) Quantitative PCR Visualizing Plasmids Lactose Repressor Protein LacO Sequence
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