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Immunology and Infection

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JoVE Journal Immunology and Infection
Flowcytometrisk Isolering av Primary Murine Type II alveolære epitelceller for Funksjonell og molekylær studier
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Flowcytometrisk Isolering av Primary Murine Type II alveolære epitelceller for Funksjonell og molekylær studier

Article DOI: 10.3791/4322-v • 14:48 min • December 26th, 2012
December 26th, 2012 •
1,2, 1,2, 3, 2, 1,2, 1
1Research Group Immune Regulation, Helmholtz Centre for Infection Research, 2Research Group Infection Immunology, Institute of Medical Microbiology, Otto-von-Guericke University, 3Department of Experimental Immunology, Helmholtz Centre for Infection Research

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Vi beskriver den raske isolasjon av primære murine type II alveolære epitelceller (AECII) etter flowcytometrisk negativ seleksjon. Disse AECII viser høy levedyktighet og renhet, og er egnet for et bredt spekter av funksjonelle og molekylære studier innen sin respiratoriske tilstander som autoimmune eller smittsomme sykdommer.

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Immunologi cellebiologi molekylær biologi Infeksjon smittsomme sykdommer mikrobiologi alveolar type II epitelceller mus luftveier lunger celle sortering flowcytometri influensa autoimmunitet
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