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Imaging Analysis of Neuron to Glia Interaction in Microfluidic Culture Platform (MCP)-based Neuronal Axon and Glia Co-culture System

Article DOI: 10.3791/4448-v • 09:34 min • October 14th, 2012
October 14th, 2012 •
1, 1,2
1Department of Neuroscience, Tufts University, 2Neuroscience Program, Tufts Sackler School of Graduate Biomedical Sciences

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This study describes the procedures of setting up a novel neuronal axon and (astro)glia co-culture platform. In this co-culture system, manipulation of direct interaction between a single axon (and single glial cell) becomes feasible, allowing mechanistic analysis of the mutual neuron to glial signaling.

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Imaging Analysis Neuron Glia Interaction Microfluidic Culture Platform MCP-based Neuronal Axon And Glia Co-culture System Signaling Pathways Central Nervous System (CNS) Neuron And Glia Mixed Cultures Neuronal Compartments Axon Dendrite Soma Culture System Soluble Factors Direct Membrane Contact Signals Single Axon Glial Cell
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