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Direct Imaging of ER Calcium with Targeted-Esterase Induced Dye Loading (TED)

Article DOI: 10.3791/50317-v • 09:32 min • May 7th, 2013
May 7th, 2013 •
1, 1,2, 1, 1, 3, 1
1Institute for Clinical Neurobiology, University of Wuerzburg, 2Department of Synapses - Circuits - Plasticity, Max Planck Institute of Neurobiology, Martinsried, 3Walter Brendel Centre of Experimental Medicine, Ludwig-Maximilians University of Munich

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Targeted-esterase induced dye loading (TED) supports the analysis of intracellular calcium store dynamics by fluorescence imaging. The method bases on targeting of a recombinant Carboxylesterase to the endoplasmic reticulum (ER), where it improves the local unmasking of synthetic low-affinity Ca2+ indicator dyes in the ER lumen.

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ER Calcium Imaging Targeted-esterase Induced Dye Loading (TED) Fluorescent Ca2+ Indicators ER Lumen Calcium Dynamics Cell Physiology Synthetic Dyes Cell Lines Glial Cells Neurons Carboxylesterase Activity Red Fluorescent Protein Two-color Imaging Lentivirus Transduction Live Cell Imaging Acetoxymethyl Ester (AM-ester) Low-affinity Ca2+ Indicators
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