Strategies for Tracking Anastasis, A Cell Survival Phenomenon that Reverses Apoptosis

Ho Lam Tang; Ho Man Tang; J. Marie Hardwick; Ming Chiu Fung

doi:10.3791/51964

Stratégies pour le suivi Anastasis, une cellule de survie Phénomène qui renverse apoptose

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Strategies for Tracking Anastasis, A Cell Survival Phenomenon that Reverses Apoptosis

Stratégies pour le suivi Anastasis, une cellule de survie Phénomène qui renverse apoptose

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12:55 min

February 16, 2015

DOI: 10.3791/51964-v

Ho Lam Tang^1,2,3, Ho Man Tang^1,2,3, J. Marie Hardwick¹, Ming Chiu Fung²

¹W. Harry Feinstone Department of Molecular Microbiology and Immunology,Johns Hopkins University Bloomberg School of Public Health, ²School of Life Sciences,Chinese University of Hong Kong, ³Center for Cell Dynamics, Department of Biological Chemistry,Johns Hopkins University School of Medicine

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Overview

Anastasis is the process by which dying cells reverse apoptosis and survive. This article presents protocols for detecting and tracking cells that undergo this phenomenon using live cell microscopy.

Key Study Components

Area of Science

Cell Biology
Apoptosis
Live Cell Imaging

Background

Anastasis allows cells to recover from programmed cell death.
Understanding this process can provide insights into cell survival mechanisms.
Live cell microscopy is essential for observing dynamic cellular changes.
Distinct morphological features differentiate healthy and apoptotic cells.

Purpose of Study

To develop protocols for detecting anastasis in cultured cells.
To track the reversal of apoptosis in real-time.
To enhance understanding of cellular recovery processes.

Methods Used

Plating cells in glass bottom culture dishes.
Live cell staining for mitochondria and nuclei.
Utilizing microscopy to observe morphological changes.
Tracking cell behavior over time.

Main Results

Successful detection of morphological features in apoptotic cells.
Tracking of cells that undergo anastasis.
Demonstration of the recovery process in live cells.
Insights into the mechanisms of cell survival.

Conclusions

Anastasis is a reversible process that can be tracked in real-time.
Live cell microscopy is a valuable tool for studying cell death and survival.
Further research may uncover therapeutic applications for cell recovery.

Frequently Asked Questions

What is anastasis?

Anastasis is the process by which dying cells reverse apoptosis and survive.

How can anastasis be detected?

Anastasis can be detected using live cell microscopy and specific staining techniques.

What are the key morphological features of apoptotic cells?

Apoptotic cells exhibit unique morphological changes that can be identified through microscopy.

Why is live cell imaging important?

Live cell imaging allows researchers to observe dynamic processes in real-time, providing insights into cellular behavior.

What implications does this research have?

Understanding anastasis may lead to new therapeutic strategies for enhancing cell survival.

Le terme anastase fait référence au phénomène dans lequel les cellules mourantes inversent un processus de suicide cellulaire à un stade avancé, se réparent elles-mêmes et finissent par survivre. Nous démontrons ici des protocoles de détection et de suivi des cellules qui subissent une anastase.

L’objectif global de cette procédure est de détecter et de suivre l’inversion du processus de mort cellulaire apoptotique et des cellules cultivées par microscopie à cellules vivantes. Les cellules saines se propagent sur le substrat et contiennent des mitochondries filamenteuses tubulaires autour du noyau. Cependant, lorsque les cellules se suicident par apoptose, les cellules mourantes présentent des caractéristiques morphologiques uniques pour détecter et suivre l’inversion de l’apoptose.

Les cellules sont plaquées dans des boîtes de culture cellulaire à fond de verre. L’étape suivante consiste à effectuer une coloration de cellules vivantes pour marquer les mitochondries et les noyaux. Cela permet de détecter les caractéristiques morphologiques des cellules saines et apoptotiques.

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Biologie cellulaire Numéro 96 Anastasis l'apoptose des corps apoptotiques caspase la mort cellulaire retrait de la cellule cellule suicide cytochrome c lésions de l'ADN des altérations génétiques mitochondrial perméabilisation de la membrane externe (MOMP) la mort cellulaire programmée inversion de l'apoptose