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Isolation and Culture of Dissociated Sensory Neurons From Chick Embryos

Article DOI: 10.3791/51991-v • 11:16 min • September 24th, 2014
September 24th, 2014 •
1, 1, 1
1Department of Natural Sciences, Assumption College

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Cell culture models provide detailed control over environmental conditions and thus provide a powerful platform to elucidate numerous aspects of neuronal cell biology. We describe a rapid, inexpensive, and reliable method to isolate, dissociate, and culture sensory neurons from chick embryos. Details of substrata preparation and immunocytochemistry are also provided.

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Isolation Dissociation Culture Sensory Neurons Chick Embryos In Vitro Cell Culture Models Environmental Conditions Variables Primary Neurons Rapid Inexpensive Robust Growth Enriched Cultures Non-neuronal Cells Laminin-containing Substrata Neuronal Plating Cellular Techniques Molecular Techniques Co-immunoprecipitation Live Cell Imaging RNAi Immunocytochemistry Double Immunocytochemistry
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