An Ex vivo Model of an Oligodendrocyte-directed T-Cell Attack in Acute Brain Slices

Kerstin G&#246;bel; Stefan Bittner; Manuela Cerina; Alexander M. Herrmann; Heinz Wiendl; Sven G. Meuth

doi:10.3791/52205

Journal

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Immunologie et infection

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Une Ex vivo Modèle de T-Cell Attaque de Oligodendrocyte dirigée dans aigus tranches cérébrales

An <em>Ex vivo</em> Model of an Oligodendrocyte-directed T-Cell Attack in Acute Brain Slices

Journal JoVE
Immunologie et infection

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Journal JoVE Immunologie et infection

An Ex vivo Model of an Oligodendrocyte-directed T-Cell Attack in Acute Brain Slices

Une Ex vivo Modèle de T-Cell Attaque de Oligodendrocyte dirigée dans aigus tranches cérébrales

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7,019 Views

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06:36 min

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February 05, 2015

DOI:

10.3791/52205-v

DOI:

10.3791/52205-v

•

06:36 min

February 05, 2015

•

6979 Views

Kerstin Göbel¹, Stefan Bittner^1,2, Manuela Cerina³, Alexander M. Herrmann¹, Heinz Wiendl¹, Sven G. Meuth^1,3

¹Department of Neurology,University of Münster ²Germany and Interdisciplinary Center for Clinical Research (IZKF) Münster ³Institute of Physiology I – Neuropathophysiology I,University of Münster

Summary

To address mechanisms of demyelination and neuronal apoptosis in cortical lesions of inflammatory demyelinating disorders, different animal models are used. We here describe an ex vivo approach by using oligodendrocyte-specific CD8⁺ T-cells on brain slices, resulting in oligodendroglial and neuronal death. Potential applications and limitations of the model are discussed.