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Intravital Imaging of Axonal Interactions with Microglia and Macrophages in a Mouse Dorsal Column Crush Injury

Article DOI: 10.3791/52228-v • 08:43 min • November 23rd, 2014
November 23rd, 2014 •
1, 2, 3, 3
1Department of Neurosciences, Case Western Reserve University, 2Department of Biomedical Engineering, Case Western Reserve University, 3Department of Pediatrics, Case Western Reserve University

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Two-photon intravital imaging can be used to investigate interactions among different cell types in the spinal cord in their native tissue environment in a bone marrow chimeric animal with a dorsal column traumatic spinal cord crush injury.

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Intravital Imaging Axonal Interactions Microglia Macrophages Mouse Dorsal Column Crush Injury Two-photon Microscopy Inflammatory Reaction Spinal Cord Injury Central Nervous System (CNS) Living Animal Distinguishing Macrophages From Microglia Genetically Encoded Green Fluorescent Protein Injury Model Laminectomy Cellular Interactions
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