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Leprdb Mouse Model of Type 2 Diabetes: Pancreatic Islet Isolation and Live-cell 2-Photon Imaging Of Intact Islets

Article DOI: 10.3791/52632-v • 10:09 min • May 11th, 2015
May 11th, 2015 •
1, 1, 1
1School of Biomedical Sciences, The University of Queensland

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We present here a protocol for the isolation of islets from the mouse model of type 2 diabetes, Leprdb and details of a live-cell assay for measurement of insulin secretion from intact islets that utilizes 2 photon microscopy.

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Type 2 Diabetes Leprdb Mouse Model Pancreatic Islet Isolation Live-cell Imaging 2-photon Imaging Beta-cell Dysfunction Research Progress Islet Isolation Protocol Wild Type Mice Diabetic Leprdb (db/db) Mice Insulin Secretion Control
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