Transfecter des cellules RAW264.7 avec un gène rapporteur de la luciférase
20,593 Views
•
12:58 min
•
June 18, 2015
DOI:
Summary
Automatically generated
Transfection into the macrophage cell line, RAW264.7, is difficult due to the cell’s natural response against foreign materials. We described here a gentle yet robust procedure for transfecting luciferase reporter genes into RAW264.7 cells.
Vidéos Connexes
07:33
In vivo Dual Substrate Bioluminescent Imaging
Vidéos Connexes
15428 Views
07:16
Monitoring Hippo Signaling Pathway Activity Using a Luciferase-based Large Tumor Suppressor (LATS) Biosensor
Vidéos Connexes
19557 Views
13:48
Studying Membrane Biogenesis with a Luciferase-Based Reporter Gene Assay
Vidéos Connexes
12788 Views
11:32
Identification of Transcription Factor Regulators using Medium-Throughput Screening of Arrayed Libraries and a Dual-Luciferase-Based Reporter
Vidéos Connexes
6678 Views
06:52
Monitoring Breast Cancer Growth and Metastatic Colony Formation in Mice using Bioluminescence
Vidéos Connexes
2957 Views
09:17
Bioluminescence Imaging of an Immunocompetent Animal Model for Glioblastoma
Vidéos Connexes
14751 Views
08:55
In vitro Transcription and Capping of Gaussia Luciferase mRNA Followed by HeLa Cell Transfection
Vidéos Connexes
18166 Views
10:38
Monitoring Cell-autonomous Circadian Clock Rhythms of Gene Expression Using Luciferase Bioluminescence Reporters
Vidéos Connexes
22253 Views
12:55
High-throughput Functional Screening using a Homemade Dual-glow Luciferase Assay
Vidéos Connexes
23453 Views
10:33
Flexible Measurement of Bioluminescent Reporters Using an Automated Longitudinal Luciferase Imaging Gas- and Temperature-optimized Recorder (ALLIGATOR)
Vidéos Connexes
7793 Views
Read Article
Cite this Article
Cheung, S. T., Shakibakho, S., So, E. Y., Mui, A. L. Transfecting RAW264.7 Cells with a Luciferase Reporter Gene. J. Vis. Exp. (100), e52807, doi:10.3791/52807 (2015).
Copy