Co-immunoprécipitation de la protéine souris Mx1 avec le virus Influenza A nucléoprotéine
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April 21, 2015
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This co-immunoprecipitation protocol allows to study the interaction between the influenza A virus nucleoprotein and the antiviral Mx1 protein in human cells. The protocol emphasizes the importance of N-ethylmaleimide for successful co-immunoprecipitation of Mx1 and influenza A virus nucleoprotein.
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Verhelst, J., De Vlieger, D., Saelens, X. Co-immunoprecipitation of the Mouse Mx1 Protein with the Influenza A Virus Nucleoprotein. J. Vis. Exp. (98), e52871, doi:10.3791/52871 (2015).
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