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Dissolution Dynamic Nuclear Polarization Instrumentation for Real-time Enzymatic Reaction Rate Measurements by NMR

Article DOI: 10.3791/53548-v • 10:54 min • February 23rd, 2016
February 23rd, 2016 •
1, 1, 2, 3, 3, 1
1LCBPT - UMR8601, Institut de Chimie, Université Paris Descartes, 2Institute of Physics of Biological Systems, EPFL, 3PARCC - INSERM U970, Université Paris Descartes

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The sensitivity enhancement provided by dissolution dynamic nuclear polarization (DNP) enables following metabolic processes in real time by NMR and MRI. The characteristics and performances of a dedicated dissolution DNP setup designed for study enzymatic reactions are discussed.

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Dissolution Dynamic Nuclear Polarization Enzymatic Reaction Rate Measurements NMR Dynamic Nuclear Polarization DNP Equipment Real-time Enzymatic Processes Liquid-state Magnetic Resonance Pair Of Molecules Dissolution DNP Equipment Sensitivity Enhancement 500 Megahertz NMR Spectrometers France's First DNP Equipment Installation Substrate Quantification Time Resolution
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