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Bioengineering

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JoVE Journal Bioengineering
Real Time Monitoring of Intracellular Bile Acid Dynamics Using a Genetically Encoded FRET-based Bile Acid Sensor
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Real Time Monitoring of Intracellular Bile Acid Dynamics Using a Genetically Encoded FRET-based Bile Acid Sensor

Article DOI: 10.3791/53659-v • 09:21 min • January 4th, 2016
January 4th, 2016 •
1, 2,3, 1
1Tytgat Institute for Liver and Intestinal Research, Department of Gastroenterology and Hepatology, Academic Medical Center, 2Laboratory of Chemical Biology, Institute of Complex Molecular Systems (ICMS), 3Department of Biomedical Engineering, Eindhoven University of Technology

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résumé
Automatic Translation

We provide a detailed protocol to study bile acid dynamics in living cells using a genetically encoded BAS FRET sensor. This Bile Acid Sensor represents a unique tool to study (regulation of) bile acid transport and FXR activation in a wide range of cell types.

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Genetically Encoded FRET-based Bile Acid Sensor Real-time Monitoring Intracellular Bile Acid Dynamics Spacial Resolution Temporal Resolution Metabolic Questions Glucose Metabolism Lipid Metabolism Energy Metabolism Single Living Cells Labeling Bile Acids Cell Transport And Dynamics Protein Localization FXR Nuclear Bile Acid Receptor Ligand Binding Domain Cerulean Fluorophore Citrine Fluorophore Inter-molecular Interactions Nuclear Localization Signal
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