L'étude de l'Organisation supramoléculaire de photosynthétiques Membranes dans les tissus foliaires Gel fracturés par Cryo-microscopie électronique à balayage
Studying the Supramolecular Organization of Photosynthetic Membranes within Freeze-fractured Leaf Tissues by Cryo-scanning Electron Microscopy
L'étude de l'Organisation supramoléculaire de photosynthétiques Membranes dans les tissus foliaires Gel fracturés par Cryo-microscopie électronique à balayage
Here we describe a procedure for studying freeze-fractured plant tissues. High-pressure frozen leaf samples are freeze-fractured and double-layer coated, yielding well preserved frozen-hydrated samples that are imaged using the cryo-scanning electron microscope at high magnifications with minimal beam damage.
Charuvi, D., Nevo, R., Kaplan-Ashiri, I., Shimoni, E., Reich, Z. Studying the Supramolecular Organization of Photosynthetic Membranes within Freeze-fractured Leaf Tissues by Cryo-scanning Electron Microscopy. J. Vis. Exp. (112), e54066, doi:10.3791/54066 (2016).