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Developmental Biology

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JoVE Journal Developmental Biology
Expansion and Adipogenesis Induction of Adipocyte Progenitors from Perivascular Adipose Tissue Isolated by Magnetic Activated Cell Sorting
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Expansion and Adipogenesis Induction of Adipocyte Progenitors from Perivascular Adipose Tissue Isolated by Magnetic Activated Cell Sorting

Article DOI: 10.3791/55818-v • 08:28 min • June 30th, 2017
June 30th, 2017 •
1, 2, 2, 1
1Department of Large Animal Clinical Sciences, Michigan State University, 2Department of Pharmacology and Toxicology, Michigan State University

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Here we report a method for isolation of Adipocyte Progenitor Cell (APC) populations from Perivascular Adipose Tissue (PVAT) using Magnetic-activated Cell Sorting (MCS). This method allows for an increased isolation of APC per gram of adipose tissue when compared to Fluorescence-Activated Cell Sorting (FACS).

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Adipocyte Progenitors Perivascular Adipose Tissue Magnetic Activated Cell Sorting Differentiation Protocols Mature Adipocytes Isolation Technique Antigen-presenting Cells Fluorescence-activated Cell Sorting Rat Surgery Abdominal Cavity Mesenteric Resistance Vessels Thoracic Aorta Fat Pads Krebs-Ringer Bicarbonate Buffer
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