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Preparation and Metabolic Assay of 3-dimensional Spheroid Co-cultures of Pancreatic Cancer Cells and Fibroblasts
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Preparation and Metabolic Assay of 3-dimensional Spheroid Co-cultures of Pancreatic Cancer Cells and Fibroblasts

Article DOI: 10.3791/56081-v • 10:51 min • August 23rd, 2017
August 23rd, 2017 •
1, 1, 2, 2, 1, 1
1Molecular Medicine Division, Translational Genomics Research Institute, 2Agilent Technologies

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Here, a method is described for the preparation of 3-dimensional (3D) spheroid co-culture of pancreatic cancer cells and fibroblasts, followed by measurement of metabolic functions using an extracellular flux analyzer.

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3-dimensional Spheroid Co-cultures Pancreatic Cancer Cells Fibroblasts Metabolic Assay Extra-cellular Flux Analyser Tumor Microenvironment Drug Sensitivity Aseptic Tissue Culture Technique T75 Flasks Growth Medium Trypsin EDTA Single Cell Suspensions Cell Counting Nanoshuttle
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