JoVE Journal
Developmental Biology
Developmental Biology
Ce contenu est en libre accès.
Chapitres
résumé
This protocol describes the isolation, culture, and calcification of rat-derived valve interstitial cells, a highly physiological in vitro model of calcific aortic valve disease (CAVD). Exploitation of this rat model facilitates CAVD research in exploring the cell and molecular mechanisms that underlie this complex pathological process.