Dendrimer-based Uneven Nanopatterns to Locally Control Surface Adhesiveness: A Method to Direct Chondrogenic Differentiation

Ignasi Casanellas; Anna Lagunas; Iro Tsintzou; Yolanda Vida; Daniel Collado; Ezequiel P&#233;rez-Inestrosa; Cristina Rodr&#237;guez-Pereira; Joana Magalhaes; Pau Gorostiza; Jos&#233; A. Andrades; Jos&#233; Becerra; Josep Samitier

doi:10.3791/56347

Nanopatterns inégale dendrimère-basé à localement gouvernes adhésivité : une méthode pour diriger...

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Bioengineering
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JoVE Journal Bioengineering

Dendrimer-based Uneven Nanopatterns to Locally Control Surface Adhesiveness: A Method to Direct Chondrogenic Differentiation

Nanopatterns inégale dendrimère-basé à localement gouvernes adhésivité : une méthode pour diriger la différenciation chondrogéniques

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14:46 min

January 20, 2018

DOI: 10.3791/56347-v

Ignasi Casanellas^1,2, Anna Lagunas^3,1, Iro Tsintzou¹, Yolanda Vida^4,5, Daniel Collado^4,5, Ezequiel Pérez-Inestrosa^4,5, Cristina Rodríguez-Pereira⁶, Joana Magalhaes^3,6, Pau Gorostiza^1,3,7, José A. Andrades^8,3, José Becerra^8,3,5, Josep Samitier^1,3,2

¹Institute for Bioengineering of Catalonia (IBEC), The Barcelona Institute of Science and Technology (BIST), ²Department of Engineering Electronics,University of Barcelona (UB), ³Networking Biomedical Research Center (CIBER), ⁴Instituto de Investigacin Biomédica de Málaga (IBIMA), Department of Organic Chemistry,Universidad de Málaga (UMA), ⁵Andalusian Centre for Nanomedicine and Biotechnology-BIONAND, ⁶Unidad de Bioingeniería Tisular y Terapia Celular (GBTTC-CHUAC), Grupo de Reumatolog ía, Instituto de Investigación Biomèdica de A Coruña (INIBIC), Complexo Hospitalario Universitario de A Coruña (CHUAC), Sergas,Universidade da Coruña (UDC), ⁷Institució Catalana de Recerca i Estudis Avançats (ICREA), ⁸Instituto de Investigación Biomédica de Málaga (IBIMA), Department of Cell Biology, Genetics and Physiology,Universidad de Málaga (UMA)

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Overview

This article describes a method for obtaining dendrimer-based uneven nanopatterns that allow for nanoscale control of local arginine-glycine-aspartic acid (RGD) surface density. The technique is applied to study cell adhesion and chondrogenic differentiation.

Key Study Components

Area of Science

Neuroscience
Cell Biology
Materials Science

Background

Dendrimer-based nanopatterns can control surface adhesiveness at the nanoscale.
RGD peptides are known to enhance cell adhesion.
Understanding cell adhesion is crucial for tissue engineering.
Chondrogenic differentiation is important for cartilage repair.

Purpose of Study

To develop a method for creating uneven nanopatterns using dendrimers.
To investigate the effects of RGD surface density on cell adhesion.
To explore the implications for chondrogenic differentiation.

Methods Used

Creation of dendrimer-based uneven nanopatterns.
Modification of first generation polynanomers with RGD peptides.
Characterization of nanopatterns using scanning probe microscopy techniques.
Analysis of cell adhesion and differentiation through microscopy and immunostaining.

Main Results

Successful creation of uneven nanopatterns that control surface adhesiveness.
Demonstrated the impact of RGD density on cell adhesion.
Provided insights into chondrogenic differentiation processes.
Microscopy and immunostaining confirmed findings.

Conclusions

Dendrimer-based nanopatterns are effective for studying cell behavior.
Local control of RGD density can influence cell adhesion and differentiation.
This method has potential applications in tissue engineering and regenerative medicine.

Frequently Asked Questions

What are dendrimer-based nanopatterns?

Dendrimer-based nanopatterns are surface structures created using dendrimers that allow for precise control over surface properties at the nanoscale.

How does RGD influence cell adhesion?

RGD is a peptide that promotes cell adhesion by interacting with specific cell surface receptors, enhancing the attachment of cells to surfaces.

What techniques are used to characterize the nanopatterns?

Scanning probe microscopy techniques are used to characterize the created nanopatterns.

Why is chondrogenic differentiation important?

Chondrogenic differentiation is crucial for cartilage repair and regeneration, making it significant in tissue engineering applications.

What role does immunostaining play in this study?

Immunostaining is used to visualize specific proteins and assess the differentiation of cells in response to the nanopatterns.

Une méthode pour obtenir nanopatterns inégale dendrimère-basé qui permettent le contrôle de l’échelle nanométrique d’arginine-glycine-aspartique acide (RGD) surface densité locale est décrite et appliquée pour l’étude de la différenciation cellulaire adhésion et chondrogéniques.

L’objectif global de ce protocole est d’obtenir des nanomotifs inégaux à grande échelle basés sur des dendrimères qui permettent le contrôle à l’échelle nanométrique de l’adhérence de surface locale. La méthode décrite est appliquée à l’étude de l’adhésion cellulaire et de la différenciation chondrogénique. Les nanomotifs en relief du dendrimère permettent de contrôler localement l’adhérence de surface à l’échelle nanométrique.

Des polynanomères de première génération modifiés avec le peptide RGD adhésif cellulaire sont utilisés pour créer des nanomotifs. Ils sont caractérisés par des techniques de balayage par microscopie et utilisés dans les études d’adhésion et de différenciation cellulaires. Nous analysons ensuite les résultats des techniques de microscopie et d’immunomarquage.

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