Establishing Mouse Models for Zika Virus-induced Neurological Disorders Using Intracerebral Injection Strategies: Embryonic, Neonatal, and Adult

Stephanie A. Herrlinger; Qiang Shao; Li Ma; Melinda Brindley; Jian-Fu Chen

doi:10.3791/56486

Établir des modèles de souris pour troubles neurologiques Zika Viro-induite à l’aide de stratégie...

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JoVE Journal Neuroscience

Establishing Mouse Models for Zika Virus-induced Neurological Disorders Using Intracerebral Injection Strategies: Embryonic, Neonatal, and Adult

Établir des modèles de souris pour troubles neurologiques Zika Viro-induite à l’aide de stratégies d’Injection intracérébrale : embryonnaires, néonatals et adultes

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09:39 min

April 26, 2018

DOI: 10.3791/56486-v

Stephanie A. Herrlinger¹, Qiang Shao², Li Ma², Melinda Brindley³, Jian-Fu Chen²

¹Biomedical and Health Sciences Institute,University of Georgia, ²Center for Craniofacial Molecular Biology,University of Southern California, ³Department of Infectious Diseases, College of Veterinary Medicine,University of Georgia

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Overview

This study presents a method for creating in vivo models of Zika virus infection in the brain using a mouse model. The primary focus is on understanding the pathology and mechanisms of Zika virus-induced microcephaly at various developmental stages, which has implications for therapies and diagnostics.

Key Study Components

Area of Science

Neuroscience
Virology
In vivo models

Background

Zika virus infection is linked to microcephaly in fetuses.
Understanding viral mechanics in the brain is crucial for addressing neurodevelopmental disorders.
This technique can also extend to other flavivirus infections, like dengue.

Purpose of Study

To establish a reliable method for studying Zika virus infections in the brain.
To investigate the effects of the virus at embryonic, neonatal, and adult stages.
To explore implications for therapy and diagnosis of associated disorders.

Methods Used

A surgical method for intracerebral inoculation of Zika virus in mouse models.
The primary biological model involves injecting embryos, neonatal pups, and adult mice.
Important steps include preparing injection syringes, anesthetizing subjects, and injecting Zika virus into specified brain regions.
Monitoring recovery and ensuring sterile conditions during surgical procedures are emphasized.

Main Results

The technique allows for controlled infection of the brain, facilitating detailed study of viral pathogenesis.
Significant insights into the development of neurodevelopmental issues related to Zika infection are anticipated.
Potentially applicable to other flavivirus models enhances the method's versatility.

Conclusions

This study establishes a foundational protocol for investigating Zika virus impact on brain development.
Insights gained may enable advancements in understanding and treating Zika-related disorders.

Frequently Asked Questions

What are the advantages of this model for studying Zika virus?

The model allows precise control of viral inoculation in various developmental stages and can yield insights into the pathology of Zika virus infections.

How is the biological model implemented?

The model involves injecting Zika virus into the brains of embryos, neonatal pups, or adult mice to study the disease's progression and effects.

What types of data are obtained from this method?

Data obtained may include viral load, developmental outcomes, and neurodevelopmental changes associated with Zika infection.

What are the limitations of this method?

Considerations include the invasive nature of the procedure and ensuring anesthetic adequacy during surgery to minimize risks to the animals.

How can this technique be adapted for other infections?

By modifying virus strains used or adjusting injection protocols, this method can be adapted to study other flavivirus infections, such as dengue.

What is the significance of studying Zika virus in the brain?

Understanding the viral mechanisms in the brain is crucial for developing interventions to address microcephaly and other neurodevelopmental issues associated with infection.

Nous décrivons ici une méthode pour établir un modèle de Zika Viro-induite microcéphalie chez la souris. Ce protocole inclut des méthodes pour l’inoculation intracérébrale embryonnaire, néonatale et adulte-stade du virus Zika.

L’objectif global des méthodes chirurgicales présentées ici est d’établir des modèles in vivo d’infection intracrânienne par le virus Zika. Cette méthode peut aider à répondre à des questions clés sur le terrain, telles que la pathologie et le mécanisme de l’infection par le virus Zika dans le cerveau à la fois au stade précoce et ultérieur du développement ainsi qu’au stade adulte. Le principal avantage de cette technique est que l’infection de direction du cerveau peut facilement être réalisée.

Les implications de cette technique s’étendent aux thérapies ou aux diagnostics de microcéphalie induite par le virus Zika ou d’autres troubles neurodéveloppementaux. Bien que cette méthode puisse donner un aperçu de la pathogenèse du virus Zika, elle peut également être appliquée pour étudier d’autres infections à flavivirus dans le cerveau, comme le virus de la dengue. Juste avant l’intervention chirurgicale, chargez le mélange d’injection du virus Zika dans une aiguille d’injection en verre préparée en assemblant d’abord la seringue d’injection étanche au gaz de 50 microlitres, l’accessoire Luer-lock et la tubulure.

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