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Journal / Genetics / Gelişmiş genom Cas9 Ribonükleoprotein yılında…
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Genetics

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JoVE Journal Genetics
Gelişmiş genom Cas9 Ribonükleoprotein yılında çeşitli ile hücreleri ve organizmaların düzenleme
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Gelişmiş genom Cas9 Ribonükleoprotein yılında çeşitli ile hücreleri ve organizmaların düzenleme

Article DOI: 10.3791/57350-v • 09:51 min • May 25th, 2018
May 25th, 2018 •
*1,2, *1, *3,4,5,6, *1,3, 1,3, 3,5,6,7,8,9, 1,2, 1,10, 3
1Department of Molecular Cell Biology, University of California, Berkeley, 2Howard Hughes Medical Institute, University of California, Berkeley, 3Innovative Genomics Institute, University of California, Berkeley, 4Biomedical Sciences Graduate Program, University of California, San Francisco, 5Department of Microbiology and Immunology, University of California, San Francisco, 6Diabetes Center, University of California, San Francisco, 7Chan Zuckerberg Biohub, 8Department of Medicine, University of California, San Francisco, 9UCSF Helen Diller Family Comprehensive Cancer Center, University of California, San Francisco, 10Department of Integrative Biology, University of California, Berkeley
* These authors contributed equally

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Preassembled Cas9 kullanan karmaşık Ribonükleoprotein (RNP) kesin, verimli genom düzenleme için güçlü bir yöntemdir. Burada, biz yararını hücreleri ve organizmalar, geniş aralığında birincil insan hücreleri ve her iki klasik de dahil olmak üzere ve model organizmalar gelişmekte olan vurgulayın.

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Genetik sorunu 135 CRISPR Cas9 gRNA RNP genom düzenleme gen düzenleme kapalı-hedefleri HDR HSPCs T hücreleri Caenorhabditis elegans Parhyale hawaiensis
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