Double-stranded RNA Oral Delivery Methods to Induce RNA Interference in Phloem and Plant-sap-feeding Hemipteran Insects

Saikat Kumar B. Ghosh; Wayne B. Hunter; Alexis L. Park; Dawn E. Gundersen-Rindal

doi:10.3791/57390

Méthodes de prestation orale ARN double-brin d’induire l’interférence d’ARN dans le phloème et in...

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Double-stranded RNA Oral Delivery Methods to Induce RNA Interference in Phloem and Plant-sap-feeding Hemipteran Insects

Méthodes de prestation orale ARN double-brin d’induire l’interférence d’ARN dans le phloème et insectes hémiptères plante-sap-alimentation

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10:14 min

May 4, 2018

DOI: 10.3791/57390-v

Saikat Kumar B. Ghosh¹, Wayne B. Hunter², Alexis L. Park¹, Dawn E. Gundersen-Rindal¹

¹Invasive Insect Biocontrol and Behavior Laboratory, Agricultural Research Service,United States Department of Agriculture, ²Horticultural Research Laboratory, Agricultural Research Service,United States Department of Agriculture

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Overview

This article demonstrates novel techniques developed for oral delivery of double-stranded RNA (dsRNA) through the vascular tissues of plants for RNA interference (RNAi) in phloem sap feeding insects. The methods focus on delivering gene-specific dsRNAs to induce RNA interference in vivo and control insect pests.

Key Study Components

Area of Science

Plant biotechnology
Insect pest management
RNA interference

Background

RNA interference is a powerful tool for gene silencing.
Delivering biomolecules to insects presents significant challenges.
Hemipteran pests are major agricultural threats.
Novel delivery methods can enhance biopesticide efficacy.

Purpose of Study

To demonstrate a natural oral delivery method for dsRNA.
To elucidate the effects of RNAi-based molecular biopesticides.
To explore delivery methods applicable to various insect types.

Methods Used

Development of techniques for dsRNA delivery through plant vascular tissues.
Application of dsRNA to hemipteran pests.
Assessment of gene silencing effectiveness in vivo.
Evaluation of the method's applicability to other insect species.

Main Results

Successful delivery of dsRNA to target insect pests.
Induction of RNA interference observed in treated insects.
Potential for broader application to other sap-feeding and chewing insects.
Demonstrated effectiveness of RNAi-based biopesticides.

Conclusions

The oral delivery method shows promise for pest control.
RNAi can be effectively utilized in agricultural practices.
Further research is needed to optimize delivery techniques.

Frequently Asked Questions

What is RNA interference?

RNA interference (RNAi) is a biological process in which RNA molecules inhibit gene expression or translation, effectively silencing targeted genes.

How does the oral delivery method work?

The method involves delivering double-stranded RNA through plant vascular tissues, allowing it to reach sap-feeding insects effectively.

What types of insects can this method target?

The techniques demonstrated can target hemipteran pests, such as the brown marmorated stink bug and the Asian citrus psyllid, and may be applicable to other insect types.

What are the advantages of using RNAi-based biopesticides?

RNAi-based biopesticides can provide a targeted approach to pest control, reducing the impact on non-target organisms and the environment.

Is this method environmentally friendly?

Yes, RNAi-based methods can be more environmentally friendly compared to traditional chemical pesticides, as they specifically target pest genes.

What further research is needed?

Further research is needed to optimize the delivery techniques and assess the long-term effects of RNAi-based biopesticides in various agricultural settings.

Cet article explique les nouvelles techniques mises au point pour l’administration orale de l’ARN double brin (dsRNA) à travers les tissus vasculaires des plantes pour l’interférence ARN (ARNi) en sève nourrir les insectes.

L’objectif global de ces procédures est de démontrer une méthode d’administration orale naturelle. Pour les ARNdb spécifiques aux gènes, pour induire une interférence ARN in vivo et les insectes ravageurs. Cette méthode peut aider à répondre à des questions clés sur la façon de délivrer des biomolécules à l’intérieur des insectes, à élucider l’effet des biopesticides moléculaires à base d’ARNi et à contrôler les insectes dans l’environnement.

Le principal avantage de cette technique est que cette administration par le biais d’un silençage génique médié par les acides nucléiques peut être appliquée à l’alimentation de la sève des plantes ainsi qu’aux insectes broyeurs. Bien que les méthodes ici démontrent l’administration d’ARN double brin aux hémiptères, comme la punaise marbrée, la punaise arlequin et le psylle asiatique des agrumes. Ils peuvent être appliqués à d’autres insectes.

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