Détection des petits GTPase prénylation et GTP Binding utilisant le fractionnement Membrane et GTPase-linked Immunosorbent Assay

9.6K vues

Cité 6 fois

13:51 min

11 novembre 2018

10.3791/57646-v

11 novembre 2018

9.6K vues

Javad Alizadeh¹^,² , Shahla Shojaei¹^,²^,³ , Simone da Silva Rosa¹ , Adel Rezaei Moghadam¹^,² , Amir A. Zeki⁴ , Mohammad Hashemi⁵ , Marek J. Los⁶^,⁷^,⁸ , Joseph W. Gordon¹^,⁹ , Saeid Ghavami¹^,²^,¹⁰

¹Department of Human Anatomy and Cell Science, Rady Faculty of Health Sciences, Max Rady College of Medicine, University of Manitoba, ²Biology of Breathing Theme, Children Hospital Research Institute of Manitoba, University of Manitoba, ³Department of Biochemistry, School of Pharmacy and Pharmaceutical Sciences, Isfahan University of Medical Sciences, ⁴Division of Pulmonary, Critical Care, and Sleep Medicine, Department of Internal Medicine, Center for Comparative Respiratory Biology and Medicine, ⁵Department of Clinical Biochemistry, School of Medicine, Zahedan University of Medical Sciences, ⁶Department of Molecular Biology, School of Pharmacy with the Division of Laboratory Medicine in Sosnowiec, Medical University of Silesia, ⁷Centre de biophysique moléculaire - UPR 4301, Centre national de la recherche scientifique (CNRS) CS80054, ⁸LinkoCare Life Sciences AB, ⁹College of Nursing and Children's Hospital Research Institute of Manitoba, Rady Faculty of Health Sciences, University of Manitoba, ¹⁰Health Policy Research Center, Institute of Health, Shiraz University of Medical Sciences

Nous décrivons ici un protocole afin d’étudier la prénylation et la guanosine-5'-triphosphate (GTP)-chargement de la Rho GTPase. Ce protocole comprend deux méthodes détaillées, à savoir fractionnement membrane et une GTPase-immuno assay. Le protocole peut être utilisé pour mesurer la prénylation et la GTP chargement de différentes autres petites GTPases.