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Biochemistry

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JoVE Journal Biochemistry
Isolation of Physiologically Active Thylakoids and Their Use in Energy-Dependent Protein Transport Assays
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Isolation of Physiologically Active Thylakoids and Their Use in Energy-Dependent Protein Transport Assays

Article DOI: 10.3791/58393-v • 12:25 min • September 28th, 2018
September 28th, 2018 •
1, 1, 1, 1
1Department of Plant Biology, University of California - Davis

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We present protocols herein for high-yield isolation of physiologically active thylakoids and protein transport assays for the chloroplast twin arginine translocation (cpTat), secretory (cpSec1), and signal recognition particle (cpSRP) pathways.

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Thylakoids Protein Transport Assays Mechanism Energetics Location Of Proteins Control Of Environment Temperature PH Ionic Conditions Precursor Concentrations Peas Distilled Water Plastic Tray Vermiculite Growth Period Chloroplast Isolation Continuous Density Gradient Percoll 2xGB Buffer Centrifugation Pea Shoots 1xGB Buffer Blender Homogenization Filtration Cheese Cloth Centrifuge Tube
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