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JoVE Journal Neuroscience
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Beredning av akut ryggmärgen skivor för hela-cell Patch-clamp inspelning i Substantia Gelatinosa nervceller

Article DOI: 10.3791/58479-v • 08:30 min • January 18th, 2019
January 18th, 2019 •
1, 1, 2, 2, 2, 2, 2,3
1Department of Pain, First Affiliated Hospital of Nanchang University, 2Department of Pediatrics, First Affiliated Hospital of Nanchang University, 3Center for Laboratory Medicine, First Affiliated Hospital of Nanchang University

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Här beskriver vi de väsentliga steg för hela-cell patch-clamp-inspelningar som gjorts från substantia gelatinosa (SG) nervceller i ryggmärgen in vitro- segmentet. Denna metod tillåter inneboende membran egenskaper, synaptisk transmission och morfologisk karakterisering av SG nervceller att studeras.

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Neurovetenskap fråga 143 neurovetenskap ryggmärgen slice substantia gelatinosa neuron hela-cell patch-clamp elektrofysiologi morfologi in vitro
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