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A Zebrafish Embryo Model for In Vivo Visualization and Intravital Analysis of Biomaterial-associated Staphylococcus aureus Infection
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1Department of Medical Microbiology,Amsterdam UMC, 2Technical Medical Center, Department of Biomaterials Science and Technology,University of Twente, 3Department of Biomedical Engineering, W.J. Kolff Institute, University Medical Center Groningen,University of Groningen, 4Institute of Biology,Leiden University
Chapitres
- 00:04Titre
- 00:44“Bacteria-only” and Bacteria-microspheres Suspension Preparation
- 03:04Zebrafish Embryo Injection
- 05:50Bacterial Culture Quantification and Infection Progression Monitoring
- 07:37Results: Representative Intravital Biomaterial-associated Staphylococcus aureus Infection Analysis
- 09:17Conclusion
The present study describes a zebrafish embryo model for in vivo visualization and intravital analysis of biomaterial-associated infection over time based on fluorescence microscopy. This model is a promising system complementing mammalian animal models such as mouse models for studying biomaterial-associated infections in vivo.
