Identification des souris et des répertoires d’anticorps humain par séquençage de prochaine génération

14K vues

Cité par 4

08:51 min

15 mars 2019

10.3791/58804-v

15 mars 2019

14K vues

Lin Sun¹ , Naoko Kono² , Hiroyuki Toh³ , Hanbing Xue¹ , Kaori Sano⁴^,⁵ , Tadaki Suzuki⁴ , Akira Ainai⁴ , Yasuko Orba⁶ , Junya Yamagishi⁷^,⁸ , Hideki Hasegawa⁴^,⁵ , Yoshimasa Takahashi⁹ , Shigeyuki Itamura² , Kazuo Ohnishi⁹^,¹⁰

¹Graduate School of Life and Environmental Sciences, University of Tsukuba, ²Center for Influenza Virus Research, National Institute of Infectious Diseases, ³School of Science and Technology, Kwansei Gakuin University, ⁴Department of Pathology, National Institute of Infectious Diseases, ⁵Division of Infectious Diseases Pathology, Department of Global Infectious Diseases, Tohoku University Graduate School of Medicine, ⁶Division of Molecular Pathobiology, Research Center for Zoonosis Control, Hokkaido University, ⁷Division of Collaboration and Education, Research Center for Zoonosis Control, Hokkaido University, ⁸Global Station for Zoonosis Control, GI-CoRE, Hokkaido University, ⁹Department of Immunology, National Institute of Infectious Diseases, ¹⁰Faculty of Life and Environmental Sciences, University of Tsukuba

Nous décrivons ici les protocoles pour l’analyse et la visualisation de la structure et la constitution de répertoires entiers d’anticorps. Il s’agit de l’acquisition de vastes séquences d’ARN d’anticorps moyen du séquençage de prochaine génération.