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Neuroscience

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JoVE Journal Neuroscience
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In vivo direkte omprogrammering af residente Gliale celler til Interneuroner ved intracerebral injektion af virale vektorer

Article DOI: 10.3791/59465-v • 12:26 min • June 17th, 2019
June 17th, 2019 •
1, 1, 1
1Department of Experimental Medical Sciences and Lund Stem Cell Center, BMC, Lund University

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Denne protokol har til formål at generere direkte omprogrammeret interneuroner in vivo, ved hjælp af et AAV-baseret viral system i hjernen og en FLEX synapsin-drevet GFP reporter, som giver mulighed for celle identifikation og yderligere analyse in vivo.

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Neurovidenskab direkte konvertering hel-celle patch clamp FLEX vektorsystem CRE-inducerbar system Skive-optagelser parvalbumin Trans differentiering neurale konvertering
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