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Genetics

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CRISPR/Cas9 Ribonucleoprotein-mediated Precise Gene Editing by Tube Electroporation
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CRISPR/Cas9 Ribonucleoprotein-mediated Precise Gene Editing by Tube Electroporation

Article DOI: 10.3791/59512-v • 08:31 min • June 20th, 2019
June 20th, 2019 •
*1, *1, 2, 1, 1, 1, 1, 1
1Center for Advanced Models for Translational Sciences and Therapeutics, Department of Internal Medicine, University of Michigan Medical Center, 2Celetrix LLC
* These authors contributed equally

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Presented here is a protocol for efficient CRISPR/Cas9 ribonucleoprotein-mediated gene editing in mammalian cells using tube electroporation.

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CRISPR/Cas9 Ribonucleoprotein Gene Editing Tube Electroporation Cellular Survival Rates Clinical Applications Virus-free Drug-free Reporter Enrichment-free Cell Types GFP Expressing Plasmid DNA Postdoc IPSCs Extracellular Matrix Feeder-free Cell Culture Medium Rho-associated Coiled-coil Containing Protein Kinase Inhibitor Y-27632
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