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Developmental Biology

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JoVE Journal Developmental Biology
Serum fri produksjon av tredimensjonale humant Hepatospheres fra Pluripotent stamceller
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Serum fri produksjon av tredimensjonale humant Hepatospheres fra Pluripotent stamceller

Article DOI: 10.3791/59965-v • 06:57 min • July 20th, 2019
July 20th, 2019 •
1, 1,2, 1,3, 1,4, 1, 1, 4, 3, 1
1MRC Centre for Regenerative Medicine, University of Edinburgh, 2UCL Great Ormond Street Institute of Child Health, University College London, 3Division of Biosciences, Department of Life Sciences, College of Health and Life Sciences, Brunel University London, 4School of Biochemistry and Immunology, Trinity Biomedical Sciences Institute, Trinity College Dublin

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Denne protokollen beskriver en tilnærming til å produsere hepatospheres fra menneskelige Pluripotent stamceller ved hjelp av et definert kultur system og celle selv montering. Denne protokollen er reproduserbar i en rekke cellelinjer, kostnadseffektive og tillater produksjon av stabile menneskelige hepatospheres for biomedisinsk anvendelse.

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Utviklingsbiologi Pluripotent stamceller tredimensjonal kultur hepatospheres stoff metabolisme og protein sekresjon stabil celle fenotype definert kultur system
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