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OaAEP1-Mediated Enzymatic Synthesis and Immobilization of Polymerized Protein for Single-Molecule Force Spectroscopy

Article DOI: 10.3791/60774-v • 08:34 min • February 5th, 2020
February 5th, 2020 •
1, 1, 1, 1, 1, 1, 1
1State Key Laboratory of Coordination Chemistry, School of Chemistry and Chemical Engineering, Nanjing University

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Here, we present a protocol to conjugate protein monomer by enzymes forming protein polymer with a controlled sequence and immobilize it on the surface for single-molecule force spectroscopy studies.

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OaAEP1-Mediated Enzymatic Synthesis Immobilization Polymerized Protein Single-molecule Force Spectroscopy Sample Preparation Protein-based Material Construction Cysteine Functional Residues Polymerized Matter Protein Enzymes Yibing Deng Shengchao Shi Grad Students Potassium Chromate Ultrapure Water Concentrated Sulfuric Acid Glass Coverslip Chromic Acid Oven 80 Degrees Celsius APTES Toluene Solution Room Temperature Pipette
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