Waiting
Traitement de la connexion…

Trial ends in Request Full Access Tell Your Colleague About Jove
Journal / Bioengineering / Preclinical Cardiac Electrophysiology Assessment…
JoVE Journal
Bioengineering

Un abonnement à JoVE est nécessaire pour afficher ce contenu.

JoVE Journal Bioengineering
Preclinical Cardiac Electrophysiology Assessment by Dual Voltage and Calcium Optical Mapping of Human Organotypic Cardiac Slices
In Vitro 3D Cell-Cultured Arterial Models for Studying Vascular Drug Targeting Under Flow
Journal (Bioengineering)
In Vitro 3D Cell-Cultured Arterial Models for Studying Vascular Drug Targeting Under Flow
Advancing Coral Culture — Creating a Semi-Quantitatively Controlled Microenvironment System to Counter Current Limitations (Video) | JoVE
Journal (Bioengineering)
Advancing Coral Culture — Creating a Semi-Quantitatively Controlled Microenvironment System to Counter Current Limitations (Video) | JoVE
 

Preclinical Cardiac Electrophysiology Assessment by Dual Voltage and Calcium Optical Mapping of Human Organotypic Cardiac Slices

Article DOI: 10.3791/60781-v • 09:35 min • June 16th, 2020
June 16th, 2020 •
*1, *1, 1
1Department of Biomedical Engineering, The George Washington University
* These authors contributed equally

Chapitres

résumé
Automatic Translation

This protocol describes the procedure for sectioning and culturing human cardiac slices for preclinical drug testing and details the use of optical mapping for recording transmembrane voltage and intracellular calcium signals simultaneously from these slices.

Tags

Preclinical Cardiac Electrophysiology Assessment Dual Voltage Calcium Optical Mapping Human Organotypic Cardiac Slices Donor Human Hearts Physiological Drug Testing Voltage Dyes Calcium Dyes Optical Mapping System Tissue Bath Polydimethylsiloxane Gel Layer Perfusion System Recovery Solution Oxygenation Temperature Maintenance Bath Perfusate Accumulation CMOS Cameras Green LED Light Source Excitation Wavelength Voltage Sensitive Dyes Calcium Indicator Dyes Tissue Sections Cardioplegia Solution Left Ventricular Free Wall Agarose Gel Vibratome
Read Article

Get cutting-edge science videos from JoVE sent straight to your inbox every month.

Waiting X
Simple Hit Counter