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Capillary Electrophoresis Mass Spectrometry Approaches for Characterization of the Protein and Metabolite Corona Acquired by Nanomaterials
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Chimie
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Journal JoVE Chimie
Capillary Electrophoresis Mass Spectrometry Approaches for Characterization of the Protein and Metabolite Corona Acquired by Nanomaterials

Capillary Electrophoresis Mass Spectrometry Approaches for Characterization of the Protein and Metabolite Corona Acquired by Nanomaterials

DOI:
10.3791/61760-v

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07:54 min

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October 27, 2020

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, , , , , ,
1School of Geography Earth and Environmental Sciences,University of Birmingham, 2Biomedical Microscale Analytics,Leiden University, 3Institute of Medical Biochemistry,Medical University of Innsbruck, 4AB Sciex UK Ltd

Chapitres

  • 00:04Introduction
  • 00:30Biomolecular Corona Formation and Protein Corona Isolation
  • 02:52Metabolite Corona Isolation
  • 03:50Preparation of CESI Capillaries and CE-MS for Protein Corona Analysis
  • 05:11Preparation of CESI Capillaries and CESI-MS for Metabolite Corona Analysis
  • 06:27Results: Mass Spectral Analysis of the Characterization of the Protein and Metabolite Corona Acquired by Nanomaterials
  • 07:26Conclusion

Summary

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Here we present a protocol to characterize the complete biomolecular corona, proteins, and metabolites, acquired by nanomaterials from biofluids using a capillary electrophoresis – mass spectrometry approach.

Tags

Capillary ElectrophoresisMass SpectrometryCESI-MSProtein CoronaMetabolite CoronaNanomaterialsHuman PlasmaProtein CharacterizationMetabolite CharacterizationTrypsin DigestionC18 DesaltingSample Preparation

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