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Immunology and Infection

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Purificación y expansión de células T asesinas naturales invariantes de ratón para estudios in vitro e in vivo

Article DOI: 10.3791/62214-v • 08:37 min • February 15th, 2021
February 15th, 2021 •
1, *1,2, *1,2, 1,2
1Experimental Immunology Unit, Division of Immunology, Transplantation and Infectious Diseases, IRCCS San Raffaele Scientific Institute, 2Vita-Salute San Raffaele University
* These authors contributed equally

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Describimos un protocolo rápido y robusto para enriquecer células T asesinas naturales invariantes (iNKT) del bazo de ratón y expandirlas in vitro a números adecuados para estudios in vitro e in vivo.

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Inmunología e Infección Número 168 células iNKT expansión ratón Vα14 CD1d perlas anti-CD3/CD28 activación linfocitos
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