Waiting
Traitement de la connexion…

Trial ends in Request Full Access Tell Your Colleague About Jove
Journal / Neuroscience / Isolation of Adult Human Astrocyte Populations…
JoVE Journal
Neuroscience

Un abonnement à JoVE est nécessaire pour afficher ce contenu.

JoVE Journal Neuroscience
Isolation of Adult Human Astrocyte Populations from Fresh-Frozen Cortex Using Fluorescence-Activated Nuclei Sorting
Laser-guided Neuronal Tracing In Brain Explants
Journal (Neuroscience)
Laser-guided Neuronal Tracing In Brain Explants
Intact Histological Characterization of Brain-implanted Microdevices and Surrounding Tissue
Journal (Neuroscience)
Intact Histological Characterization of Brain-implanted Microdevices and Surrounding Tissue
 

Isolation of Adult Human Astrocyte Populations from Fresh-Frozen Cortex Using Fluorescence-Activated Nuclei Sorting

Article DOI: 10.3791/62405-v • 08:18 min • April 16th, 2021
April 16th, 2021 •
1,2, 1,2, 3, 2,4, 1,2
1Department of Pathology, Icahn School of Medicine at Mount Sinai, 2Department of Neuroscience and Friedman Brain Institute, Icahn School of Medicine at Mount Sinai, 3Institutes of Brian Science, Fudan University, 4Department of Psychiatry, Icahn School of Medicine at Mount Sinai

Chapitres

résumé
Automatic Translation

We have developed a method that enriches for and isolates human astrocyte populations from fresh-frozen tissue for use in downstream molecular analyses.

Tags

Isolation Adult Human Astrocyte Populations Fresh-frozen Cortex Fluorescence-activated Nuclei Sorting Molecular Function Specific Cell Type Astrocytes Bulk Human Brain Tissue Specimens Fluorescent-conjugated Antibodies Neocortex Neurons Oligodendrocyte Progenitor Cells Dissecting Tissue Douncer Lysis Buffer Sucrose Buffer Ultracentrifuge Tube Nuclei Pel
Read Article

Get cutting-edge science videos from JoVE sent straight to your inbox every month.

Waiting X
Simple Hit Counter