JoVE Journal
Biology
Biology
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Chapters
Summary
Please note that all translations are automatically generated.
该协议描述了在外科主动脉瓣置换手术期间或从尸体组织中提取的人主动脉瓣的收集,以及随后对患者特异性原发性瓣膜内皮和间质细胞的分离、扩增和表征。其中包括有关确保细胞活力和表型特异性所需过程的重要细节。