Waiting
Traitement de la connexion…

Trial ends in Request Full Access Tell Your Colleague About Jove
Journal / Immunology and Infection / 流式细胞术分析人髓系U937细胞中SAMHD1限制性…
JoVE Journal
Immunology and Infection

Ce contenu est en libre accès.

JoVE Journal Immunology and Infection
流式细胞术分析人髓系U937细胞中SAMHD1限制性
Chemoselective Modification of Viral Surfaces via Bioorthogonal Click Chemistry
Journal (Immunology and Infection)
Chemoselective Modification of Viral Surfaces via Bioorthogonal Click Chemistry
Zika Virus Infectious Cell Culture System and the In Vitro Prophylactic Effect of Interferons
Journal (Immunology and Infection)
Zika Virus Infectious Cell Culture System and the In Vitro Prophylactic Effect of Interferons
 
Click here for the English version

流式细胞术分析人髓系U937细胞中SAMHD1限制性

Article DOI: 10.3791/62502-v • 09:43 min • June 13th, 2021
June 13th, 2021 •
1, 2, 1, 3
1Retrovirus-Host Interactions Laboratory, The Francis Crick Institute, 2Retroviral Replication Laboratory, The Francis Crick Institute, 3Sidney Sussex College, Department of Medicine, University of Cambridge

Chapitres

résumé
Automatic Translation

Please note that all translations are automatically generated.

Click here for the English version.

这里描述的是一种确定HIV-1限制程度的既定方法，由细胞抑制蛋白SAMHD1。人髓系U937细胞用共表达YFP的SAMHD1表达载体转导，分化，然后用HIV-RFP攻击。限制水平由流式细胞术分析确定。

Tags

免疫学和感染，第172期，人类免疫缺陷病毒-1型（HIV-1），SAMHD1，限制性，骨髓细胞，流式细胞术，逆转录病毒
Read Article

Get cutting-edge science videos from JoVE sent straight to your inbox every month.

Waiting X
Simple Hit Counter