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Innsamling, utvidelse og differensiering av primære humane neseepitelcellemodeller for kvantifisering av cilia beat frekvens

Article DOI: 10.3791/63090-v • 11:13 min • November 10th, 2021
November 10th, 2021 •
1,2, 1,2, 1,2,3, 1,2, 1,2,3, 4, 5, 1,2,3
1School of Women's and Children's Health, Faculty of Medicine and Health, University of New South Wales, 2Molecular and Integrative Cystic Fibrosis Research Centre (miCF RC), Faculty of Medicine and Health, University of New South Wales, 3Department of Respiratory Medicine, Sydney Children's Hospital, 4Department of Pathology, School of Medical Sciences, University of New South Wales Australia, 5Katharina Gaus Light Microscopy Facility, Mark Wainwright Analytical Centre, University of New South Wales

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Denne protokollen beskriver nasal epitelcelleinnsamling, utvidelse og differensiering til organotypiske luftveisepitelcellemodeller og kvantifisering av cilia beat-frekvens via live-celleavbildning og spesialbygde skript.

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