Waiting
Traitement de la connexion…

Trial ends in Request Full Access Tell Your Colleague About Jove
Journal / Biochemistry / Generating Self-Assembling Human Heart Organoids…
JoVE Journal
Biochemistry

Un abonnement à JoVE est nécessaire pour afficher ce contenu.

JoVE Journal Biochemistry
Generating Self-Assembling Human Heart Organoids Derived from Pluripotent Stem Cells
Bioluminescent Optogenetics 2.0: Harnessing Bioluminescence to Activate Photosensory Proteins In Vitro and In Vivo
Journal (Biochemistry)
Bioluminescent Optogenetics 2.0: Harnessing Bioluminescence to Activate Photosensory Proteins In Vitro and In Vivo
Visualization of Estrogen Receptors in Colons of Mice with TNBS-Induced Crohn's Disease using Immunofluorescence
Journal (Biochemistry)
Visualization of Estrogen Receptors in Colons of Mice with TNBS-Induced Crohn's Disease using Immunofluorescence
 

Generating Self-Assembling Human Heart Organoids Derived from Pluripotent Stem Cells

Article DOI: 10.3791/63097-v • 08:56 min • September 15th, 2021
September 15th, 2021 •
1,2, 1,2, 1,2, 1,2, 1,2
1Institute for Quantitative Health Science and Engineering, Division of Developmental and Stem Cell Biology, Michigan State University, 2Department of Biomedical Engineering, College of Engineering, Michigan State University

Chapitres

résumé
Automatic Translation

Here, we describe a protocol to create developmentally relevant human heart organoids (hHOs) efficiently using human pluripotent stem cells by self-organization. The protocol relies on the sequential activation of developmental cues and produces highly complex, functionally relevant human heart tissues.

Tags

Self-assembling Human Heart Organoids Pluripotent Stem Cells 3D Structure Developing Human Heart Cost-effective Reproducible Differentiation Steps Cell Types Chambers Vascular Network Modeling Cardiovascular Diseases Pharmacological Treatments High Throughput Congenital Heart Defects Cell Culture Skills Embryoid Bodies Detachment PSC Medium Single Cell Suspension
Read Article

Get cutting-edge science videos from JoVE sent straight to your inbox every month.

Waiting X
Simple Hit Counter