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Scalable Isolation and Purification of Extracellular Vesicles from Escherichia coli and Other Bacteria

Article DOI: 10.3791/63155-v • 09:56 min • October 13th, 2021
October 13th, 2021 •
1,2,3, 1, 1,4, 5, 1, 1,3, 1,3,4
1Department of Cardiovascular & Metabolic Sciences, Lerner Research Institute, Cleveland Clinic, 2University Hospitals Cleveland Medical Center, 3Case Western Reserve University, 4Center for Microbiome & Human Health, Cleveland Clinic, 5Electron Microscopy Core, Lerner Research Institute, Cleveland Clinic

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Bacteria secrete nanometer-sized extracellular vesicles (EVs) carrying bioactive biological molecules. EV research focuses on understanding their biogenesis, role in microbe-microbe and host-microbe interactions and disease, as well as their potential therapeutic applications. A workflow for scalable isolation of EVs from various bacteria is presented to facilitate standardization of EV research.

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Scalable Isolation Purification Extracellular Vesicles Escherichia Coli Bacteria Reproducible Manner In Vivo Studies Preclinical Applications Therapeutics Scalable Cell Culture System Filters SEC Columns Starting Volumes Bacterial Cell Culture Sadie Johnson Assistant Technologist Dr. Justin Lathia's Lab Dionysius Watson Oncology Fellow Akeem Santos Research Technologist Inoculating Single Colonies Luria-Bertani Broth LB Broth Sterile Loop Incubation Shaking Incubator Rotations Per Minute 37 Degrees Celsius Bacterial Culture Medium Polypropylene Centrifuge Bottles
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