Waiting
Traitement de la connexion…

Trial ends in Request Full Access Tell Your Colleague About Jove
Journal / Biochemistry / An Optimized Single-Molecule Pull-Down Assay for…
JoVE Journal
Biochemistry

Un abonnement à JoVE est nécessaire pour afficher ce contenu. Connectez-vous ou commencez votre essai gratuit.

JoVE Journal Biochemistry
An Optimized Single-Molecule Pull-Down Assay for Quantification of Protein Phosphorylation
A Mass Spectrometry-Based Proteomics Approach for Global and High-Confidence Protein R-Methylation Analysis
Journal (Biochemistry)
A Mass Spectrometry-Based Proteomics Approach for Global and High-Confidence Protein R-Methylation Analysis
Small-Scale Colorimetric Assays of Intracellular Lactate and Pyruvate in the Nematode Caenorhabditis elegans
Journal (Biochemistry)
Small-Scale Colorimetric Assays of Intracellular Lactate and Pyruvate in the Nematode Caenorhabditis elegans
 

An Optimized Single-Molecule Pull-Down Assay for Quantification of Protein Phosphorylation

Article DOI: 10.3791/63665-v • 07:45 min • June 6th, 2022
June 6th, 2022 •
1, 1, 1, 2, 2, 1, 2,3, 1,3
1Department of Pathology, University of New Mexico School of Medicine, 2Department of Physics and Astronomy, University of New Mexico, 3Comprehensive Cancer Center, University of New Mexico Health Sciences Center

Chapitres

résumé
Automatic Translation

The present protocol describes sample preparation and data analysis to quantify protein phosphorylation using an improved single-molecule pull-down (SiMPull) assay.

Tags

SiMPull Optimized Single-molecule Pull-down Assay Quantification Protein Phosphorylation Antibody Labeling Fixation Conditions Autofluorescence Reduction Single Molecule Analysis Algorithms Phosphorylation Status Novel Information Western Blotting Mass Spec Analysis Researchers Demonstration Julian Rojo Piranha Etching Cover Glass Coating Array Drawing And Sample Imaging
Read Article

Get cutting-edge science videos from JoVE sent straight to your inbox every month.

Waiting X
Simple Hit Counter