This protocol presents an accessible guide for collecting, storing, and thawing peripheral blood mononuclear cells suitable for downstream analyses and workflows like flow cytometry and RNA sequencing. Plasma and buffy coat collections are also demonstrated.
Dinh, B., Hoeksema, M. A., Spann, N. J., Rendler, J., Cobo, I., Glass, C. K., Yeang, C. Isolation and Cryopreservation of Highly Viable Human Peripheral Blood Mononuclear Cells From Whole Blood: A Guide for Beginners. J. Vis. Exp. (212), e66794, doi:10.3791/66794 (2024).